Description
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1 – 5 PAGER CMS
100 Assays
$285
250 Assays
$355
Whats included?
SERVICES
This kit contains reagents for routine detection of Top1 activity. DNA markers (included) allow unequivocal detection of the enzyme. The assay is specific for topoisomerase I; (eukaryotic type II topoisomerases require MgCl2 and ATP). The standard topo I assay kit is supplied with EDTA containing reaction buffer (minus Mg++) in order to prevent nucleolytic nicking of the supercoiled substrate (although we note that some eukaryotic type I activities are stimulated by Mg++). This assay will work even with crude extracts. A detailed manual of instruction (see link above) is included that describes how to run the gels to maximally resolve topoisomers for analysis of DNA unwinding.
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TECHNICAL SUPPORT
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PUBLICATIONS
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NEWS
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GRANTS
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CUSTOMIZED SUPPORT
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