Polyclonal Antibody to Human Topoisomerase II (p170) – ideal for Western blotting, immunofluorescence and immunoprecipitation
A polyclonal antibody was prepared against a 16 residue oligopeptide derived from the carboxy terminal region of human topoisomerase II. The purity of the peptide was greater than 95% as determined by HPLC and mass spectral analysis. The antibody titer was determined by ELISA to be > 1:104. Immunoaffinity gel chromatography and antibody purification steps were carried out to obtain a homogeneously purified IgG.
This antibody is ideal for either Western blotting applications or immunoflourecence (IF) studies on the cytological localization of topoisomerase II. The dilutions of antibody required for specific detection should be optimized for the investigator’s particular system (cell line being used for example); however, we supply the antibody reagent along with a detailed protocol for immunofluorescence using a typical human cell line (optimized for HeLa cells). A second reagent, the 16 -mer oligopeptide ( TG2011-2) is also available. This allows the investigator to perform proper controls for absorbing the antibody to demonstrate specificity in IF experiments. The IF protocol has been optimized for tumor cells that possess relatively high levels of topoisomerase II. In addition, we offer a Western blot marker set for topo II (TG2011-3) to unambiguously locate the position of the 170 kDa topo II.
Polyclonal Antibody Unit Definition:
The antibody concentration is in Western blotting units; one unit corresponds to a 1:1000 dilution of the antibody required to make 1 ml of diluted probe for a routine Western blot. Thus, 10 units of antibody will make 10 ml of diluted probe.
Material safety data:
THIS PRODUCE IS NOT LICENSED OR APPROVED FOR ADMINISTRATION TO HUMANS OR TO ANIMALS OTHER THAN EXPERIMENTAL ANIMALS.
The product is sold as an antibody for research purposes only. Safe lab practices should be employed when handling this product. Chemical, physical and toxicological analyses of the product have not been performed.
The antibody is shipped at ambient temperature but should be stored at -20°C.