Human Topoisomerase II Alpha Molecular Weight Marker Background
The marker set for topo II is used to unambiguously locate the position of the 170 kDa topo II in Western Blots. The human 170 kDa is present in SDS-PAGE loading buffer. Note that human topo II may break down spontaneously with prolonged storage or with repeated freezing and thawing cycles. TopoGEN, Inc. stipulates that the marker be 90% undegraded at the time of shipment.
- The presence of the 170 band is confirmed by Western blotting; less than 5-10% degradation of the 170 band is detected.
- There is no 180 kDa topo II in this preparation.
- Loading 10 ul of extract is sufficient for use as a marker in Western blotting.*
*By special order, TopoGEN can supply enough of this marker to visualize the p170 band by commasie blue staining; please enquire about Catalog Number TG2011-3C.
Material Safety Data:
THIS PRODUCT IS NOT LICENSED OR APPROVED FOR ADMINISTRATION TO HUMANS OR TO ANIMALS OTHER THAN EXPERIMENTAL ANIMALS.
The polypeptide is shipped on dry ice and is supplied in SDS-PAGE gel loading buffer. The tube should be divided into aliquots after thawing; store the aliquots at -70°C.