Genistein is an isoflavone and an inhibitor of tyrosine protein kinases. It has been reported to induce topo II dependent cleavages (Biochem. Pharmacol. 39:737-740 ). TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II (Note, Genistein may also inhibit topoisomerase I.) For in vitro cleavage detection, we recom- mend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of assay being employed (SDSK+ vs. Radioactive End Labeled DNA vs. testing for plasmid cleavages). Note that large amounts of enzyme will be required to detect cleavage complex, compared to assays that measure catalytic activity.
Chemical Name: 5,7-Dihydroxy-3-(4-hydroxyphenyl)-4H-1- benzopyran-4-one
Molecular Formula: C15H10O5
Molecular Weight: 270.23
TG4160 is supplied already suspended in DMSO at 10 mM (0.25 ml) and is ready for use. The drug is pre-tested and shown to induce topo cleavages using purified human topoisomerase from TopoGEN.
The liquid product is shipped on blue ice. Store at -20°C.