E. coli DNA Gyrase


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E. coli Bacterial DNA Gyrase Product Description

Highly purified DNA Gyrase is offered as a holoenzyme (contains both A,B subunits).  This is an excellent reagent for supercoiling plasmids in vitro or for novel drug screens.

Quality Control Tests:
Nuclease contamination: assayed by testing for linear KDNA and linear plasmid DNA formation.  Incubation of 1 µg of catenated KDNA or supercoiled pUC19 DNA for 4 hrs. at 37°C (under gyrase assay conditions and with or without ATP).

Assay Conditions:
Supercoiling assays are carried out using relaxed pBR-322 DNA under conditions specified in the protocol sheets provided with the product.  We also perform assays using kDNA as substrate.  For details on this decatenation/supercoiling assay please refer to our technical literature (link provided above). One unit of gyrase activity will supercoil 0.2 ug of plasmid in 30 min at 37°C.

Included Materials:
A 5X assay uffer and dilution buffer is included with the enzyme.  These are also sold separately as 5X Gyrase Assay Buffer (TG4030) Gyrase Dilution Buffer (please enquire).

Purification of Gyrase A and B Subunits:

Reviews and Citations

  • Phillips JW, Goetz MA, Smith SK, Zink DL, Polishook J, Onishi R, Salowe S, Wiltsie J, Allocco J, Sigmund J, Dorso K, Lee S, Skwish S, de la Cruz M, Martin J, Vicente F, Genilloud O, Lu J, Painter RE, Young K, Overbye K, Donald RGK, Singh SB: Discovery of Kibdelomycin, A Potent New Class of Bacterial Type II Topoisomerase Inhibitor by Chemical-Genetic Profiling in Staphylococcus aureus.  Cell Chemistry and Biology 2011, 18: 955-965.  doi: 10.1016/j.chembiol.2011.06.011.
Shipping and Storage Instructions:

The enzyme is shipped on dry ice and should be stored at -70°C. We also recommend that the enzyme be aliquoted after the first thaw (repeated rounds of freeze/thaw may cause loss of activity); the enzyme activity is stable for 1-3 days on ice.

Additional information

Unit Options

100 Units, 500 Units, 1000 Units, 2000 Units