Human: HeLa NHEJ Reporter Cell Line (Includes I-Sce1 Transfection)

This is a HeLa cell-based reporter kit to analyze NHEJ in live cells.  Specifically this kit will identify agents (drugs, natural products, small molecules, synthetics, miRNAs, and other genes) that affect or impact NHEJ repair. The kit uses GFP as an in situ readout for NHEJ activity in a cell context.  The NHEJ process is activated by transfecting with an I-Sce1 expression plasmid. A specific reporter based assay for NHEJ can be very beneficial for anti-cancer drug discovery projects, learning more about the process of NHEJ repair and establishing intersecting pathways, druggable pathway targets, aging and disease modeling in cells.

Customers may request receiving a reporter cell line ready for culture (shipped at ambient temperature). The cells can be stored at 4° C for no more than one week before plating. Other reagents should be stored at 4° C (short term) or -20° C (long term) upon receipt. Customers may also request frozen cells, which must be placed in ultra-low freezer (-80° C) or in liquid nitrogen storage, until ready for use.

1. HN-HeLa Cells ready for culture in T-25 flask or as a frozen cell pellet (customer may request either).  We offer frozen cells to customers who wish to store the cells for a future date of use.  We also can send live cells to customers wishing to start working immediately with the line.
2. I-Sce1 gene expression plasmid, pSCE1.
3. Neo (G418) stock for maintaining cell stocks.
4. A detailed protocol describing how to set and use this kit for assaying NHEJ mechanisms in the cell host provided.